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opera qehs high content screening microscope  (Revvity)


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    Revvity opera qehs high content screening microscope
    Pseudomonads negatively affect B. subtilis . (a) DK1042 constitutively expressing mKate2 was mixed pairwise with 720 fluorescent soil isolates and cultured for 24 h at 30°C in a microplate format. Monocultures of DK1042 (purple wells) were used for the comparison of pellicle formation, and wells with non-inoculated medium (yellow wells) were used to control for contamination. Each well was imaged in four positions with a Perkin Elmer Opera <t>QEHS,</t> acquiring images in the Z -direction every 2 µm to obtain a cube with height 40 µm. Media were removed before microscopy. (b) DK1042 biovolumes were compared between co- and monoculture to yield log 2 (fold change). Points represent medians of three replicates. Neutral, negative, and positive categories were assigned based on median and interquartile range.
    Opera Qehs High Content Screening Microscope, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 2740 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opera qehs high content screening microscope/product/Revvity
    Average 96 stars, based on 2740 article reviews
    opera qehs high content screening microscope - by Bioz Stars, 2026-05
    96/100 stars

    Images

    1) Product Images from "Taxonomy of Pseudomonas spp. determines interactions with Bacillus subtilis"

    Article Title: Taxonomy of Pseudomonas spp. determines interactions with Bacillus subtilis

    Journal: mSystems

    doi: 10.1128/msystems.00212-24

    Pseudomonads negatively affect B. subtilis . (a) DK1042 constitutively expressing mKate2 was mixed pairwise with 720 fluorescent soil isolates and cultured for 24 h at 30°C in a microplate format. Monocultures of DK1042 (purple wells) were used for the comparison of pellicle formation, and wells with non-inoculated medium (yellow wells) were used to control for contamination. Each well was imaged in four positions with a Perkin Elmer Opera QEHS, acquiring images in the Z -direction every 2 µm to obtain a cube with height 40 µm. Media were removed before microscopy. (b) DK1042 biovolumes were compared between co- and monoculture to yield log 2 (fold change). Points represent medians of three replicates. Neutral, negative, and positive categories were assigned based on median and interquartile range.
    Figure Legend Snippet: Pseudomonads negatively affect B. subtilis . (a) DK1042 constitutively expressing mKate2 was mixed pairwise with 720 fluorescent soil isolates and cultured for 24 h at 30°C in a microplate format. Monocultures of DK1042 (purple wells) were used for the comparison of pellicle formation, and wells with non-inoculated medium (yellow wells) were used to control for contamination. Each well was imaged in four positions with a Perkin Elmer Opera QEHS, acquiring images in the Z -direction every 2 µm to obtain a cube with height 40 µm. Media were removed before microscopy. (b) DK1042 biovolumes were compared between co- and monoculture to yield log 2 (fold change). Points represent medians of three replicates. Neutral, negative, and positive categories were assigned based on median and interquartile range.

    Techniques Used: Expressing, Cell Culture, Comparison, Control, Microscopy



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    Pseudomonads negatively affect B. subtilis . (a) DK1042 constitutively expressing mKate2 was mixed pairwise with 720 fluorescent soil isolates and cultured for 24 h at 30°C in a microplate format. Monocultures of DK1042 (purple wells) were used for the comparison of pellicle formation, and wells with non-inoculated medium (yellow wells) were used to control for contamination. Each well was imaged in four positions with a Perkin Elmer Opera <t>QEHS,</t> acquiring images in the Z -direction every 2 µm to obtain a cube with height 40 µm. Media were removed before microscopy. (b) DK1042 biovolumes were compared between co- and monoculture to yield log 2 (fold change). Points represent medians of three replicates. Neutral, negative, and positive categories were assigned based on median and interquartile range.
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    Image Search Results


    High-throughput microscopy imaging of yeast strains Transfer cells from 4 × 96-well deep-well blocks to an imaging plate by re-arranging them in 384-format, and image using a high-throughput confocal microscope.

    Journal: STAR Protocols

    Article Title: Protocol for cell image-based spatiotemporal proteomics in budding yeast

    doi: 10.1016/j.xpro.2024.103577

    Figure Lengend Snippet: High-throughput microscopy imaging of yeast strains Transfer cells from 4 × 96-well deep-well blocks to an imaging plate by re-arranging them in 384-format, and image using a high-throughput confocal microscope.

    Article Snippet: Spinning disk confocal microscope (Evotec Opera QEHS High-Content Screening System) , PerkinElmer , –.

    Techniques: High Throughput Screening Assay, Microscopy, Imaging

    Journal: STAR Protocols

    Article Title: Protocol for cell image-based spatiotemporal proteomics in budding yeast

    doi: 10.1016/j.xpro.2024.103577

    Figure Lengend Snippet:

    Article Snippet: Spinning disk confocal microscope (Evotec Opera QEHS High-Content Screening System) , PerkinElmer , –.

    Techniques: Recombinant, Software, Microscopy, High Content Screening, Imaging

    Pseudomonads negatively affect B. subtilis . (a) DK1042 constitutively expressing mKate2 was mixed pairwise with 720 fluorescent soil isolates and cultured for 24 h at 30°C in a microplate format. Monocultures of DK1042 (purple wells) were used for the comparison of pellicle formation, and wells with non-inoculated medium (yellow wells) were used to control for contamination. Each well was imaged in four positions with a Perkin Elmer Opera QEHS, acquiring images in the Z -direction every 2 µm to obtain a cube with height 40 µm. Media were removed before microscopy. (b) DK1042 biovolumes were compared between co- and monoculture to yield log 2 (fold change). Points represent medians of three replicates. Neutral, negative, and positive categories were assigned based on median and interquartile range.

    Journal: mSystems

    Article Title: Taxonomy of Pseudomonas spp. determines interactions with Bacillus subtilis

    doi: 10.1128/msystems.00212-24

    Figure Lengend Snippet: Pseudomonads negatively affect B. subtilis . (a) DK1042 constitutively expressing mKate2 was mixed pairwise with 720 fluorescent soil isolates and cultured for 24 h at 30°C in a microplate format. Monocultures of DK1042 (purple wells) were used for the comparison of pellicle formation, and wells with non-inoculated medium (yellow wells) were used to control for contamination. Each well was imaged in four positions with a Perkin Elmer Opera QEHS, acquiring images in the Z -direction every 2 µm to obtain a cube with height 40 µm. Media were removed before microscopy. (b) DK1042 biovolumes were compared between co- and monoculture to yield log 2 (fold change). Points represent medians of three replicates. Neutral, negative, and positive categories were assigned based on median and interquartile range.

    Article Snippet: Pellicles were incubated at 30°C for 24 h, before removing the supernatant underneath the pellicle and scanning the plate in an Opera QEHS high-content screening microscope (PerkinElmer) equipped with a UAPO20 × W3/340 objective with NA = 0.7.

    Techniques: Expressing, Cell Culture, Comparison, Control, Microscopy